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. Author manuscript; available in PMC: 2017 May 18.
Published in final edited form as: Sci Transl Med. 2016 Sep 21;8(357):357ra123. doi: 10.1126/scitranslmed.aaf2341

Fig. 6. Donor memory-like NK cells display enhanced antileukemia responses at 1 week after adoptive transfer.

Fig. 6

Freshly isolated peripheral blood mononuclear cells (PBMCs) from patient blood or BM were stimulated with K562 leukemia cells at an effector/target ratio of 10:1 for 6 hours and assessed for IFN-γ production by flow cytometry. (A) Percentage of IFN-γ – positive donor versus recipient NK cells in the peripheral blood for all patients. Data were compared using paired t test. (B) Representative flow cytometric data showing donor and recipient NK cell IFN-γ responses from theperipheralblood.(C)Absolutenumbersofrecipient(blue)anddonor(green) NK cells producing IFN-γ in the blood. (D) Summary of data from (C) depicting relative IFN-γ production by recipient and donor NK cells from the blood; the data were normalized to donor IFN-γ –positive NK cells, which were set to 100%. (E) Representative flow cytometry data showing donor and recipient NK cell IFN-γ responses from the BM. (F) Absolute numbers of recipient and donor NK cells producing IFN-γ in the BM. (G) Summary of data from (F) depicting relative IFN-γ production by recipient and donor NK cells from the BM; the data were normalized to donor IFN-γ –positive NK cells, which were set to 100%. Normalized control NK cell responses were tested against 100% (donor) using a one-sample t test. Numbers represent percentage of cells within the indicated quadrant. All summary data depict means ± SEM.