Figure 5.

Niclosamide suppresses migration and invasion of uveal melanoma cells. (A) 92.1 and Omm2.3 were cultured in 6-well plates and scratches were created using sterile pipette tips after cells reached confluence. Records were taken at the indicated times after niclosamide (2 μmol/L) treatment. Representative scratches for 92.1 and Omm2.3 (left); the breadth of scratches was measured and bar graphs (right) were the quantitative analysis of percentage of wound closure relative to the breadth of beginning from 3 random microscopic fields at the indicated time points. Data represent mean ± SD. *, p< 0.05; **, p< 0.01; ***, p< 0.001, Student's t test. (B) 92.1 and Omm2.3 cells were incubated in the presence or absence of niclosamide for 48 h, and then subjected to transwell or matrigel invasion chamber assays. The migrated or invaded cells were counted in 3 random fields. Data represent mean ± SD. *, p< 0.05; **, p< 0.01; ***, p< 0.001, Student's t test. (C) The migration and invasion related protein MMP-9 in the whole cell lysates from the 92.1 and Omm2.3 cells treated with niclosamide was detected by Western blot. (D) 92.1 cells were transduced with either lentivirus MMP-9 cDNA or MMP-9-shRNA constructs, and treated with or without niclosamide for 36 h, and then subjected to matrigel invasion chamber assays. The invaded cells were counted in 3 random fields. Data represent mean ± SD. *, p< 0.05; **, p< 0.01; ***, p< 0.001; ns, no significance, Student's t test.