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. 2017 May 18;2(10):e92920. doi: 10.1172/jci.insight.92920

Figure 2. Exacerbated pristane-induced lupus in the absence of NOX2-derived ROS.

Figure 2

(A) BALB/c.Ncf1** mice produce higher levels of lupus autoantibodies and develop aggravated renal damage. IgG autoantibodies against double-stranded DNA (dsDNA), Sm/RNP, and histone were analyzed in sera of naive and pristane-primed mice (n =  13) by ELISA. Boundaries of boxes represent 25th/75th percentiles, lines within boxes indicate medians, and whiskers represent minimum/maximum values. Significant differences after testing by 2-way ANOVA with Bonferroni’s post-hoc test were detected beginning 60 days after pristane injection. (B) Box-and-whisker plots of IgG1, IgG2a, IgG2b, IgG3, and IgM anti-dsDNA autoantibodies in sera of pristane-injected BALB/c.Ncf1** and BALB/c WT mice (n = 8). **P < 0.01, ***P < 0.001, as determined by 2-way ANOVA with Bonferroni’s post-hoc test. (C) Representative immunofluorescence images of renal IgG deposits in BALB/c WT and BALB/c.Ncf1** mice before and 4 and 6 months after pristane injection. Scale bars: 100 μm. Scatter plot shows individual values, mean, and SEM of deposits at day 180 from 8 to 10 mice. *P < 0.05 as determined by 2-tailed Student’s t test. (D) Representative periodic acid–Schiff staining of kidneys from unchallenged and pristane-injected BALB/c WT and Ncf1** mice. Dramatically worsened mesangial cell and matrix expansion at day 180 after pristane injection can be seen in BALB/c.Ncf1** mice. Scale bars: 100 μm. Scatter plot shows individual values, mean, and SEM of renal damage at day 180 (n = 4). *P < 0.05 as determined by 2-tailed Student’s t test. (E) Monthly quantification of proteinuria in BALB/c.Ncf1** and WT mice before and after pristane injection. Shown are means and SEM (n = 7). **P < 0.01, ***P < 0.001, as determined by 2-way ANOVA using Bonferroni’s post-hoc test. (F) Box-and-whisker plots showing medians, 25th/75th percentiles, and minimum/maximum values of serum cytokine/chemokine concentrations from mice 3 months after pristane injection (n = 14). **P < 0.01, ***P < 0.001 as determined by 2-tailed Mann-Whitney U test.