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. 2017 May 18;2(10):e90019. doi: 10.1172/jci.insight.90019

Figure 6. Reduced DNA methylation by depleting NNMT.

Figure 6

(A and B) SAM and L-methionine levels under transient transfection of siNNMT for 3 days in indicated GSCs. Data represent as box and whisker plot of 3 independent experiments. Differences of mean tested with 2-tailed Student’s t test. (C) 5-Methylcytosine measurement under transient transfection of siNNMT for 3 days in T4121 GSCs. 5hmC, 5-Hydroxymethylcytosine; un-mC, un-methylcytosine. Pie and Tukey’s box and whisker graphs were developed from same data for visualization. Data were represented as box and whisker plot of 3 technical replicates. Differences of mean tested with 2-tailed Student’s t test. (D) Immunoblot analysis of indicated antibodies in T4121 GSCs transduced with shCTRL, shNNMT.840, or shNNMT.330 lentivirus.