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. 2017 May 18;2(10):e88609. doi: 10.1172/jci.insight.88609

Figure 2. Analyses of neocortical development in human infants.

Figure 2

(A) Sections from the human neocortex at 26 GWs were stained with H&E (HE) and immunostained with anti-nestin antibody, anti-vimentin antibody, and anti-HuB antibody. The vimentin-positive fibers in the outer subventricular zone (OSVZ) were in bundles (arrows). CP, cortical plate; SP, subplate; IZ, intermediate zone; ISVZ, inner subventricular zone; VZ, ventricular zone. Scale bar: 200 μm. (B) High-magnification images of images in A showing the ISVZ/VZ immunostained with anti-nestin antibody, anti-vimentin antibody, and anti-Musashi1 antibody. Scale bar: 50 μm. (C) High-magnification images of the OSVZ. Scale bar: 10 μm. (D) High-magnification images of the SP. The vimentin-positive fibers in the SP were separated and contained varicosities. Cells with leading processes were present (arrows). Scale bar: 10 μm. (E and F) Sections from the human occipital neocortex at 25 GWs were stained with anti-DCX (green) and CUX2 (magenta) antibodies. High-magnification images of the cells in E (indicated by arrows) are shown in F (indicated by arrows). Scale bar: 50 μm (E); 20 μm (F). (G) Sections from the human occipital neocortex at 25 GWs were stained with anti-DCX (green) and BRN2 (magenta) antibodies. The cells with DCX-positive processes were also positive for BRN2 (indicated by arrows). Scale bar: 20 μm.