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. 2017 May 18;2(10):e88609. doi: 10.1172/jci.insight.88609

Figure 4. BRN2-positive cells were abundantly distributed in the subplate and intermediate zone of the human neocortex at 23 GWs.

Figure 4

(A) Sections from the human occipital neocortex at 23 GWs were stained with anti-BRN2 antibody (left; gray scale, right; green) and counterstained with DAPI (middle; gray scale, right; purple). (B) High-magnification images of the boxed areas in A (labeled a–d) are shown. The BRN2-positive cells accumulated at the top of the cortical plate (CP) and were diffusely distributed throughout the neocortex, including the subplate (SP) and intermediate zone (IZ). (C) High-magnification images of the squares in B (labeled i–ix) are shown. Scale bar: 200 μm (A); 50 μm (B); 10 μm (C). (D) Sections from the developing mouse neocortex from E15.5 to P1.5 were stained with an anti-Brn2 (green) antibody and counterstained with DAPI (purple). Scale bar: 200 μm. (E) The ratio of the number of Brn2-positive cells in the CP to the total number of Brn2-positive cells throughout the mouse neocortical wall was calculated (mean ± SEM; n = 4, respectively). (F) The ratio of the number of BRN2-positive cells in the CP to the total number of BRN2-positive cells throughout the human neocortical wall (23 GWs: n = 3, 25 GWs: n = 2) was calculated and plotted.