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. 2017 May 18;2(10):e87032. doi: 10.1172/jci.insight.87032

Figure 3. Depletion of unlicensed NK cells results in diminished antigen-specific CD8+ T cell numbers and viral control.

Figure 3

(A) Representative MHC-I tetramer (Tet) gating strategy and staining on CD3+CD8+Tet+ T cells for each group. Antibody depletions were performed 2 days prior to infection. (B) Absolute number of M45+CD8+ Tet+ T cells in the liver 7 days after MCMV infection in C57BL/6 (H2b) mice. (C and D) Absolute number of NP+CD8+Tet+ T cells in the lung after 7 days of APR8 infection in C57BL/6 (C) or B10.D2 (D) mice. (E) Viral loads in livers of C57BL/6 mice at day 7 after MCMV infection determined by qPCR of the IE1 gene copy number per 100 mg of tissue. (F and G) Relative PFU/ml of APR8 determined by qPCR at day 7 after infection in the lungs of C57BL/6 (F) or B10.D2 (G) mice. n = 3 or 6 mice per group, representative of 2 to 3 experiments. One-way ANOVA with Tukey post-test used to compare groups. *P < 0.05, **P < 0.01.