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. 2017 May 18;2(10):e89729. doi: 10.1172/jci.insight.89729

Figure 4. E2F1-KO HepG2 cells show increased LDL-cholesterol (LDL-C) uptake and decreased cholesterol biosynthesis.

Figure 4

(A) Relative expression of PCSK9 mRNA in HepG2 and E2F1-KO HepG2 cells. Three independent experiments in triplicate. (B) Representative images of PCSK9 Western blots from HepG2 and E2F1-KO HepG2 cells. Three independent experiments. Normalization of the quantified values is represented. (C) Representative images of LDL-C uptake in HepG2 and E2F1-KO HepG2 cells. Three independent experiments. Red fluorescence denotes LDL-C, and nuclei are stained in blue. Scale bars: 100 μm. (D) Representative images of LDLR Western blots from HepG2 and E2F1-KO HepG2 cell membranes. Three independent experiments. Ponceau staining is shown as loading control (LC). (E) Quantification of the rate of cholesterol biosynthesis in HepG2 and E2F1-KO HepG2 cells. Three independent experiments in triplicate. (F) Representative role of E2F1 in PCSK9-LDLR cholesterol uptake. All data are presented as the mean ± SEM. Differences between HepG2 and E2F1-KO HepG2 were determined by 2-tailed unpaired t test. **P < 0.01, ***P < 0.001, ****P < 0.0001.