Figure 9.

Effect of the transient inhibition of T. gondii endogenous DNMT activity with 5-AzaC A: 5-AzaC treatment of T. gondii had no apparent influence on infection rate at 2 h post infection, and the infection rates in the inhibitor treatment groups were a little bit higher than that in the control group, but no significant difference was shown (p>0.05). Error bars: SEM. B: at 48 h post infection, the average number of parasites in each PV was approximately 5 in host cells infected with normal tachyzoites; while, in the cells infected with 5-AzaC treated tachyzoites, the number of tachyzoites per PV was approximately 3; a significant difference was found between the control group and the 5-AzaC treatment group (p<0.05), but no significant difference was observed between the groups infected with the tachyzoites treated with different concentrations (12.5 µM, 100 µM) of 5-AzaC. Error bars: SEM. C: the light microscopy of the HFF cells infected by tachyzoites treated or untreated with 5-AzaC, stained with Giemsa, and observed under a 100x objective lens (1000x magnification) at 48 h post infection. In the control group, the mean number of tachyzoites per PV was significantly more than that in the 5-AzaC treatment group. Bar: 10µm