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. 2017 May 16;5:e3233. doi: 10.7717/peerj.3233

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©2017 Zhu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) and (D) show the DNA damage analysis of 100nM-TR and 100nMN-CR of Hep-2 cell line after radiation, respectively; (B) and (E) show the DNA damage analysis of 100nM-TR and 100nMN-CR of M2e cell line after radiation, respectively; (C) and (F) show the DNA damage analysis of 100nM-TR and 100nMN-CR of TU212 cell line after radiation, respectively. The appearance of “comet” with fragmented DNA (tail) being separated from undamaged nuclear DNA (head) was seen in 100nM-TR and 100nMN-CR of Hep-2, M2e, and TU212 CSCs after radiation. It was found that the “heads” of “comet” of 100nM-TR were smaller than that of 100nMN-CR, while the “tails” of “comet” of 100nM-TR were longer than that of 100nMN-CR. These data were indicated that the DNA damage of 100nM-TR were more serious than that of 100nM-CR in laryngeal CSCs after radiation.