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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: J Am Chem Soc. 2017 Jan 17;139(4):1448–1451. doi: 10.1021/jacs.6b12819

Figure 2.

Figure 2

Representative extracted-ion chromatogram (+14 charge state) of non-denaturing mass spectrometry displaying the AmpR EBD as an intact dimer and in complexes with muropeptides. The spikes in the signals represent multiple adducts of ammonium acetate from the matrix to the various species. (A) AmpR EBD (20 μM) alone. (B) AmpR EBD (20 μM) + 4 (500 μM). (C) AmpR EBD (20 μM) + 1c (500 μM). (D) AmpR EBD (20 μM) + 2c (1 mM). (E) AmpR EBD (20 μM) + 2a (1 mM). Refer to Figure S3 for additional substrate concentrations.