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(A) Structure overlay of the P. aeruginosa AmpR EBD monomer in gold (PDB code: 5MMH) with that of C. freundii bound to 4 in green (PDB code: 4WKM; no electron density for UDP was seen). The diaminopimelate (third amino acid in the stem peptide) ion-pairs with the gatekeeper R133 once R133 repositions to the open conformation. Models of P. aeruginosa AmpR EBD dimer bound to (B) 4, (C) 1c, and (D) 2c.
