Figure 5. 1-butanol did not affect spindle cortex migration.

Oocytes at GV stage were cultured for 8 h in maturation medium containing 0.1% 1-butanol, and then fixed for immunofluorescence analysis. Three replicates were included in this experiment, and at least 30 oocytes were analyzed in each group every time. DNA was stained in blue, microtubules in green and PLD2 in red. Scale bar = 20 µM. (A) Typical images illustrated the spindle was assembled in large size but positioned in the cortex after 1-butanol incubation. (B) Statistical data showed that the spindle was significantly enlarged in 1-butanol group than in control (P < 0.001). (C) Results of statistical analysis confirmed 1-brutanol had no effect on spindle migration toward the cortex.