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. 2017 May 16;5:e3295. doi: 10.7717/peerj.3295

Figure 6. Cytoplasmic distribution of actin was changed in NFOT-treated oocytes.

Figure 6

Oocytes were labeled with Alexa flour 555—phalloidin and DAPI after 8 h incubation with DMSO or NFOT. The experiment was repeated three times. DNA was visualized in blue and actin in red. Scale bar = 20 µM. (A) In DMSO group, the actin was assembled as quite thick fibers in the cytoplasm, with pretty high concentration surrounding the spindle area (arrows on the left), while in the NFOT group, fibers were taken place by the big clusters (arrows on the right). (B) Statistical analysis confirmed that the proportion of oocytes with actin clusters in cytoplasm was significantly higher in NFOT group (P < 0.001).