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. 2017 May 16;5:e3295. doi: 10.7717/peerj.3295

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©2017 Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Oocytes at GV stage were incubated for 8 h with or without NFOT, and then fixed for immunostaining of PLD2 and RhoA. This experiment was repeated three times, with 50 oocytes included in each group every time. DNA was labeled in blue, RhoA in green and PLD2 in red. Scale bar = 20 µM. (A) Immunofluorescence showed co-localization of RhoA with PLD2 was only detected in the spindle area in control cells (2–3); however, in NFOT-treated oocytes, RhoA was co-localized with PLD2 in the spindle area, but also aggregated as foci and overlapped with PLD2 dots in cytoplasm (6–8: arrows). (B) Statistical analysis confirmed the proportion of oocytes with cytoplasmic RhoA dots was significantly higher in NFOT group (P < 0.001).