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. 2017 May 18;12(5):e0177087. doi: 10.1371/journal.pone.0177087

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© 2017 Knytl et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — DAPI counter-stained metaphase spreads showed all (A) 20 chromosomes (B&W) in X. tropicalis and/or (D) 40 chromosomes in X. mellotropicalis. 28S rDNA amplicon labelled by Digoxigenin-11-dUTP (green) stained (B) XTR 7 secondary constriction and telomeres of XTR 6, 7 and 9 and/or (E) XME 7a secondary constriction and telomeres of XME 4a and 5b. 5S rDNA amplicon labelled by Biotin-16-dUTP (red) revealed positive signals on telomeric segments of (C) XTR 2, 3, 4, 5, 6, 7, 8 and 9 and/or (F) XME 4a, 5b and 8b. Arrows show NORs situated on secondary constrictions of (B) XTR 7 and (E) XME 7a. Scale bar represents 10 μm.