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. 2008 Feb 19;78(3):572–580. doi: 10.1093/cvr/cvn048

Figure 6.

Figure 6

COMP-Ang-1 enhances nitric oxide production by activating endothelial nitric oxide synthase (eNOS). (A) Nitrite levels in peripheral bloods from SHRs. (B) Nitrite levels in human umbilical vein endothelial cells (HUVEC) after the incubation with serum obtained from SHRs. Of note, pre-treatment with soluble Tie2 abolished the increase of nitrite production in HUVEC treated with serum from pCOMP-Ang-1 transferred SHRs. *P < 0.05, ***P < 0.001 vs. untreated or pLacZ; #P < 0.001 vs. pCOMP-Ang-1 (A, n = 5; B, n = 4). (C) eNOS phosphorylation in the lung tissues of pLacZ- or pCOMP-Ang-1-transferred SHRs sacrificed 3 weeks after the gene transfer. (D) eNOS phosphorylation in HUVEC after 60 min incubation with COMP-Ang-1 protein (100 ng/mL). (E) Nitrite production in HUVEC after the treatment with COMP-Ang-1 protein (100 ng/mL). *P < 0.05 vs. PBS (n = 3).