Fig 4. Knockdown of Rac3 has no effect on Rac1^loxP/loxP; ZCre⁺ oocyte maturation.

(A) Knockdown of Rac3 through RNA interference in Rac1^loxP/loxP; ZCre⁺ oocytes. The Rac1^loxP/loxP; ZCre⁺ oocytes were microinjected control SiRNA or Rac3 SiRNA, incubated in M16 medium with 2.5μM Milrinone for 24 hours, and then were collected for qRT-PCR. The experiments were repeated at least three times. ** P<0.01. (B) Normal MII eggs matured from Rac3-knockdowned- Rac1^loxP/loxP; ZCre⁺ oocytes. The control SiRNA or Rac3 SiRNA was microinjected into Rac1^loxP/loxP; ZCre⁺ oocytes. The oocytes were incubated in M16 medium with 2.5μM Milrinone for 24 hours, then were cultured in M16 medium, and the MII eggs were collected for immunofluorescent staining. The experiments were repeated for three times, and the typical images were shown. Green, spindle; Red,chromosomes.Bar,50μM.