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. 2017 May 18;12(5):e0177218. doi: 10.1371/journal.pone.0177218

Fig 4. Morphometric and histologic studies.

Fig 4

(A) Illustration of the methods used in morphometric analyses. The area enclosed by the continuous black line is the lumen area (LA), the area enclosed by internal elastic lamina (IEL), except for the LA, is the intima area (IA), and the area enclosed by the external elastic lamina (EEL) and IEL is the media area (MA). (B) Elastin-orcein stain of representative muscular pulmonary artery, scale bar = 20μm.(C) Elastin-orcein stain of representative intercostal artery, scale bar = 100 μm. The double-headed green arrows shows intimal thickening (IT) measured by the percentage of intimal area (%IA = 100X intimal area/ measured total area or area encompassed by the EEL). Note the difference between the IT of pulmonary (more remodeled arteries) and intercostal arteries.