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. Author manuscript; available in PMC: 2017 May 18.
Published in final edited form as: J Diabetes Metab. 2016 Jun 16;7(6):676. doi: 10.4172/2155-6156.1000676

Figure 7. Regulation of fibrosis-related gene expression by pre-miR-205 overexpression in HepG2 cells.

Figure 7

HepG2 hepatocytes were cultured and transfected with pre-miR-205, scrambled sequence miR or no-oligonucleotide (C-control) as described in Methods. (a) qRT-PCR quantified expression of the indicated gene transcripts and miR-205 levels at 48 hrs after transfection. (b) Western blot of HepG2 protein extracts treated as indicated for 48h and probed with SREBP1C, MMP9 and anti-actin antibodies. (c) Quantification of SREBP1 and MMP9 from western blots as in (b) normalized for actin expression. All data are mean ± SEM; * p<0.05; *** p<0.001, NS is non-significant (n=6).