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. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Biomacromolecules. 2016 Nov 11;17(12):3872–3882. doi: 10.1021/acs.biomac.6b00931

Figure 4. Effect of cell density on the formation of pancreatic cancer cell spheroids.

Figure 4

(A) Representative confocal z-stack images of live/dead stained COLO357 cells. (B) Images of F-actin/DAPI staining on day 10. (C) Cumulative distribution of cell spheroid diameter. (D) Cell viability as assessed by Alamarblue® reagent. All gel formulations contained 7 wt% GelNB, 0.9 wt% THA.