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. 2017 May 18;12(5):e0177728. doi: 10.1371/journal.pone.0177728

Table 2. Primers, cycling conditions and expected product sizes of Enterococcus AMR gene PCRs.

Resistance to: Oligo (5’–3’) Cycling conditions Products size (bp) Reference
Daptomycin liaR-F:GGTCCGATCATCCACATCTA
liaR-R:CCGTTTAGGCGTTTCATCAT
30s 94°C, 30s 60°C, 30s 72°C x 30 553 This study
liaS-F:AAAGTCATTGGTGGGGAGAA
liaS-R:GACTGGGAAGCGTTGATGAT
30s 94°C, 30s 60°C, 30s 72°C x 30 526
Tigecycline rpsJ-F:AGAGGTTGCGACACGCCCGG
rpsJ-R:TCTACAACAGTTACTGGAAT
30s 94°C, 30s 60°C, 30s 72°C x 30 525 [13]