Table 2. Primers, cycling conditions and expected product sizes of Enterococcus AMR gene PCRs.
Resistance to: | Oligo (5’–3’) | Cycling conditions | Products size (bp) | Reference |
---|---|---|---|---|
Daptomycin | liaR-F:GGTCCGATCATCCACATCTA liaR-R:CCGTTTAGGCGTTTCATCAT |
30s 94°C, 30s 60°C, 30s 72°C x 30 | 553 | This study |
liaS-F:AAAGTCATTGGTGGGGAGAA liaS-R:GACTGGGAAGCGTTGATGAT |
30s 94°C, 30s 60°C, 30s 72°C x 30 | 526 | ||
Tigecycline | rpsJ-F:AGAGGTTGCGACACGCCCGG rpsJ-R:TCTACAACAGTTACTGGAAT |
30s 94°C, 30s 60°C, 30s 72°C x 30 | 525 | [13] |