Fig 1. Absence of DdrC increases UV-sensitivity of cells devoid of the UvsE endonuclease.
A Wild type (R1), ΔddrC (GY 15929), ΔuvrA (GY 15971), ΔuvsE (GY 15972), ΔddrC ΔuvrA (GY 15973), ΔddrC ΔuvsE (GY 15974) mutant bacteria grown to an A650nm = 0.3 were serially diluted in TGY2X broth and aliquots (10 μl) of each dilution were spotted on TGY agar plates. Then, the plates were exposed to UV radiation at the indicated UV doses before incubation at 30°C for 3–5 days. B ΔuvrA ΔuvsE (GY 15977) and ΔddrC ΔuvrA ΔuvsE (GY 15978) and C ΔrecA (GY 15180) and ΔrecA ΔddrC (GY 15965) mutants were treated as described in Fig 1A. All experiments were performed at least 3 times.