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. 2017 May 11;10:2527–2538. doi: 10.2147/OTT.S134551

Figure 2.

Figure 2

Effects of miR-381 expression on growth, colony formation, and apoptosis in ESCC.

Notes: (A) qRT-PCR detection of miR-381 expression in mock or stably transfected TE10 and TE11 cells. U6 was used as an internal control. (B) MTT analysis of growth of TE10 and TE11 cells following stable transfection with pGLV3/miR-NC and pGLV3/miR-381, respectively. (C) Colony formation assay was performed. (D) Flow cytometric analysis of apoptosis in TE10 and TE11 cells stably transfected with pGLV3/miR-NC and pGLV3/miR-381, respectively. (E) Western blot detection of c-caspase-3, total caspase-3, c-PARP, and total PARP in the stably transfected TE10 and TE11 cells. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. *P<0.05 and **P<0.01 vs control.

Abbreviations: miR-381, microRNA-381; ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; NS, nonsignificant; OD, optical density; NC, negative control.