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. 2017 May 11;10:2527–2538. doi: 10.2147/OTT.S134551

Figure 3.

Figure 3

Effect of miR-381 expression on radiosensitivity of ESCC cells.

Notes: (A) Radiosensitization by expression of miR-381 was evaluated based on clonogenic cell survival assays. Stably transfected TE10 and TE11 cells were exposed to various doses of radiation prior to plating. (B) Colony formation assay was performed. (C) Flow cytometric detection of apoptosis in stably transfected TE10 and TE11 cells with or without irradiation (6.0 Gy). (D) Western blot detection of c-caspase-3, total caspase-3, cleaved c-PARP, and total PARP proteins in the stably transfected TE10 and TE11 cells with or without irradiation (6.0 Gy). GAPDH was used as an internal control. Each experiment was performed at least in triplicate. *P<0.05 and **P<0.01 vs control.

Abbreviations: miR-381, microRNA-381; ESCC, esophageal squamous cell carcinoma; NC, negative control.