Figure 4.

miR-381 binds to the 3′-UTR of XIAP mRNA.

Notes: (A) A human XIAP/3′-UTR fragment containing wild-type or mutant miR-381-binding sequence cloned downstream of the luciferase reporter gene in pLUC-luc. (B) pLUC-luc vector containing XIAP/3′-UTR-wt or XIAP/3′-UTR-mut and pGCMV/miR-381 or pGCMV/miR-NC was co-transfected into HEK-293 cells, and cell lysates were prepared at 48 h for measuring luciferase activity, which was normalized to Renilla luciferase activity. (C) Western blot detection of XIAP protein expression in the stably transfected TE10 and TE11 cells. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. **P<0.01 vs control.

Abbreviations: miR-381, microRNA-381; XIAP, X-linked inhibitor of apoptosis protein; wt, wild-type; mut, mutant; NS, nonsignificant; NC, negative control.