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. Author manuscript; available in PMC: 2017 May 18.
Published in final edited form as: Int Educ Res J. 2016 Dec;2(12):91–97.

Figure 2. Treatment with rapamycin (1μM) increases primary cilia length in epithelial and endothelial cells.

Figure 2

(A) Cells were stained with ciliary marker acetylated-α-tubulin (green) and a nuclear marker (DAPI; blue). Rapamycin treatment increased cilia length in both cell lines. Each image was compiled from different z-stack captures. (B) Cilia length was significantly longer in rapamycin-treated cells, with a two-fold increase observed in endothelial cells. N=50–70 for each slide preparation, and a total of 4 slides was used in each group. * = p<0.05 compared to corresponding controls.