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. 2017 May 18;12(5):e0177844. doi: 10.1371/journal.pone.0177844

Fig 5. Brusatol reduces cyclin B1 expression in oocytes.

Fig 5

A) The relative mRNA level of cyclin B1 was determined by qRT-PCR in control and Brusatol treated oocytes. mRNA level in control oocytes were set as 1. B) Oocytes at MI stage were immunostained with cyclin B1 antibody (green) and counterstained with Hoechst 33342 for chromosomes (blue). C) Western blot showing decline of cyclin B1 after Bruastol treatment with actin as a loading control. C) Western blot showing increase of cyclin B1 after Nrf2 overexpression with actin as a loading control. Error bars indicate ± sd Scale bar, 20 μm. *p < 0.05.