Fig 2. Selection of target sites in the VvPDS gene.

(a) Locations of target sites of CRISPR/Cas9-mediated target mutagenesis in the ‘Neo Muscat’ (V. vinifera L.) PDS gene. (b) Sequences of target sites. The PAM sequence (NGG) is shown in blue, and the 20bp target sequence is shown in black. The red arrowhead indicates the expected cleavage site. The recognition sequences of the restriction enzymes used for CAPS analysis are underlined. (c) In vitro cleavage assay of four target sites.