Table 1. Primer sequences.
Primer name | Primer sequences |
---|---|
PDS-t2F | ACCATAAAATGATTATGTAATGCAAA |
PDS-t2R | TGTCTTATAATAACCAATAAGGGGAGA |
PDS-t3F | GGCGCAAGCAATGTTGTAG |
PDS-t3R | CCAAAATCAATTTCAATGGTCA |
NPTII-F | GGCTATTCGGCTATGACTGG |
NPTII-R | CATGTGTCACGACGAGATCC |
Cas-F | TCGAGAAGATGGATGGAACC |
Cas-R | CTGAAACCTGAGCCTTCTGG |
AtU6p-F | TGTTTATCAGCTTACATTTTCTTGAACCGTAGCT |
sgRNAs-R | TAATGCCAACTTTGTACAAGAAAGCTGGGTCTAGA |
chr13-F | TGTTGATTATAATTTTTTCATATTT |
chr13-R | AAAACCAAATGTAACTGTTACTTC |
chr17-F chr17-R chr19-F chr19-R |
CCACTAAAATGTAGAAATGGGG TTCTACATCCAAGCAATTGC ATGATGAATTCATGTAAGCTGC ACCCTCTTTTCCCGACTTTC |
M13F | GTAAAACGACGGCCAG |
M13R | CAGGAAACAGCTATGA |