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. 2017 May 18;12(5):e0178219. doi: 10.1371/journal.pone.0178219

Fig 1. Expression of Slc34a2a and related transcripts during zebrafish embryogenesis.

Fig 1

(A) Schematic representation of the Slc34a2a, Slc34a2a(as) and Rbpja loci. The antisense transcript Slc34a2a(as) is depicted in red. (B) RT-qPCR analysis of Slc34a2a, Slc34a2a(as) and Rbpja transcripts including the paralog Slc34a2b. Based on negative controls using RNA as an input, the detection limit was set at a ΔCt of 12 which is in agreement with ISH results. (C) Demonstration of Slc34a2a, Slc34a2a(as), Rbpja, Slc34a2b and Shh (Sonic Hedgehog) transcripts at progressing stages of development by whole mount ISH.