Fig 1. In vitro validation and in vivo experimental design.

(A, top panel) Three rCDVs expressing a fluorescent reporter protein as an ATU from the 6^th position in the genome were generated (rCDV^SHVenus(6), rCDV^SHdTom(6), rCDV^SHTagBFP(6)), rescued and (A, bottom panel) grown on Vero-cCD150 cells. (B) rCDVs were shown to efficiently infect ConA-stimulated ferret WBC. (C) Donor ferrets 1–6 (D1-6) were simultaneously inoculated with a low dose of the three indicated rCDVs delivered via Oc, IN and IT inoculation in different administration route combinations, divided over three sequential experiments.