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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Nat Methods. 2016 Oct 24;13(12):1043–1049. doi: 10.1038/nmeth.4042

Figure 2.

Figure 2

Characterization of the dynamics and dose response of ABA-and GA-inducible gene activation. (a,b) 7-d timecourse for clonal HEK293T pTRE3G-EGFP cells stably expressing Sp or Sa sgTRE3G and (a) ABA-inducible or (b) GA-inducible VPR-Sp or Sa dCas9. Cells were continuously induced with 100 μM ABA or 10 μM GA for 7 d (ON7); induced for 2 d then cultured without inducer for 5 d (ON2 OFF5); or induced for 2 d, cultured without inducer for 3 d, then reinduced for 2 d (ON2 OFF3 ON2). The data are displayed as individual data points for two independent platings of a stable cell line (n = 2). (c,d) Dose response after 48 h induction for HEK293T pTRE3G–EGFP cells transfected with Sp or Sa sgTRE3G and (c) ABA-inducible or (d) GA-inducible VPR–Sp or Sa dCas9. The shaded regions indicate the approximate linear dose–response ranges. The data are displayed as mean ± s.d. for four independent transfections (n = 4).