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. Author manuscript; available in PMC: 2017 Nov 18.
Published in final edited form as: Oncogene. 2016 Dec 12;36(20):2868–2878. doi: 10.1038/onc.2016.440

Figure 3. miR-708 targets KPNA4 and inhibits PCa migration.

Figure 3

(a) Sequence alignment of miR-708 and KPNA4 3′UTR. KPNA4 expression in PC3 cells transfected with either (b) miR-708 mimic/negative control or (c) miR-708 inhibitor/negative control was determined by Western blotting, β-actin was used as loading control for total cell lysate. (d) HEK293T cells were co-transfected with miR-708 mimic or negative control mimic and dual-luciferase reporter plasmid inserted with KPNA4 3′UTR/control 3′UTR. Firefly luciferase was detected to determine the binding between miR-708 and KPNA4 3′UTR, renilla luciferase was used as internal control. (e) Transwell assay was performed to determine the cell invasion of PC3 cells that were transfected with either miR-708 mimic or control mimic for 48 hours.