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. Author manuscript; available in PMC: 2017 Nov 18.
Published in final edited form as: Oncogene. 2016 Dec 12;36(20):2868–2878. doi: 10.1038/onc.2016.440

Figure 6. TNF-α and β mediates the KPNA4 induced prostate cancer migration.

Figure 6

(a–b) TNF-α and TNF-β expression was evaluated in the primary tumor tissue by immunofluorenscence staining. (c) M2-phenotype associated cytokines of primary murine macrophages that were subjected to TNF-α or TNF-β stimulation (5ng/mL) was determined by real-time PCR. (d) Transwell assay of PC3-shKPNA4 or scramble control cells lines in the absence or presence of U937 cells to determine the cell invasion, PC3-shKPNA4 cells were stimulated with or without recombinant TNF-α or β cytokines (5ng/mL). (e–f) Invasive cells were quantitated by crystal violet staining assay. *p<0.05, **p<0.01.