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. 2017 May 18;7:2063. doi: 10.1038/s41598-017-02275-1

Figure 5.

Figure 5

The excitatory effect of SNP was mediated by TRPV1. (a) Response of whole nerve activity before and after SNP administration in the presence DMSO (0.1%). The upper trace shows the raw recording of whole nerve discharge, the middle trace is the number of nerve discharge in 3 s, and the lower trace shows corresponding intraluminal pressure. The arrows indicate the administration of SNP (0.16 mM). DMSO didn’t change the excitatory effect of SNP on the spontaneous afferent discharge. (b) Response of whole nerve activity to SNP in the existence of CZP (50 µM). SNP (0.16 mM) failed to increase the spontaneous afferent discharge. (c) Summary values showing that the excitatory effect of SNP (0.16 mM) was inhibited by CZP (50 µM). n = 6 in each group, *P < 0.05 vs DMSO group, Students’ t-test. (d) Response of whole nerve activity to capsaicin (0.3 µM). The application of capsaicin increased the spontaneous afferent discharge. (e) Response of whole nerve activity to capsaicin (0.3 µM) in the presence of SNP (0.16 mM). SNP markedly increased the capsaicin-evoked mesenteric afferent firing. (f) Summary values showing that the excitatory effect of capsaicin (0.3 µM) was enhanced by SNP (0.16 mM). n = 6 in each group, # P < 0.01 vs vehicle, Students’ t-test.