Figure 6.

The CD1d protein retained in late endocytic compartments. Representative images of human DCs from the gated HLA-DR⁺CD11c⁺CD1d⁺Lamp1⁺ cells are shown together with cell identities and size bars (a). Fifty cells with strong co-stain and subcellular localization of CD1d and Lamp1 proteins were extracted from the gated DC population from each of three BaP exposure conditions (none, 0.32 μM, and 5.94 μM). In this ImageJ-Fiji analysis, the co-localized pixel intensity between CD1d and Lamp1 proteins is first shown with scatterplots (b). In parallel to that in Fig. 5, Mander’s coefficients were further calculated and are shown using each column to represent an average calculation of 50 cell images extracted from each of three BaP exposure conditions (c). The percentage of co-localized pixel intensity was also calculated and is similarly shown (d). Statistical significance (n = 50, p < 0.001) was obtained with Student’s t-tests in comparison to the non-exposed group (*) and lower exposure group (**) with indicated standard errors. Data are from one experiment using cells from an independent donor. Two experiments were performed with similar results.