Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 18;7:2098. doi: 10.1038/s41598-017-02197-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ex ovo CAM assay: MUG-Mel2 onplants formed highly pigmented tumors within the silicone ring on the CAM surface after three days of incubation. Avian vessels developed radially towards the onplants (A: 10x magnification, B: 25x magnification), loco-regional metastases with solitary evading tumor cells surrounded by newly formed blood vessels were present (C: 63x magnification). Morphological analysis of hematoxylin/eosin stained sections revealed strong interaction of MUG-Mel2 cells with the CAM mesenchyme and invasion of tumor cells from the primary onplant site into the surrounding CAM tissue (D). MUG-Mel2 cells are mitotic active (Ki-67 staining) (E), tumor-driven neoangiogenesis was detected with anti-desmin (F). MUG-Mel2 cells express the tumor biomarker vimentin (G) (400x magnification, scale bar = 20 µm).