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. 2017 May 18;7:2098. doi: 10.1038/s41598-017-02197-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of MUG-Mel2 in 3D cultures. Cells formed spheroids in nanofibrillar cellulose (NFC) after two days in a concentration of 0.4% (A: 50x magnification), after five days in a concentration of 0.4% (B: 50x magnification); intensive brown staining was observed. Calcein staining (green) revealed the viability of the spheroids, nuclei were counterstained with DAPI (blue) (C). HE staining of human tumor tissue (D), tumor xenotransplantat (E), and 3D culture of MUG-Mel2 cells showed typical melanoma morphology and pigmented cytoplasm (F).