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. 2017 May 18;7:2132. doi: 10.1038/s41598-017-02264-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal scans of larval filets reveal JHR expression in 3^rd instar larval body wall tissues, including epidermis, muscle, and fat body. The expression of the Met-GAL4::p65 and gce-GAL4::p65 constructs was visualized using the nuclear reporter Stinger-GFP in (a–h). The gce-T2A-GAL4 construct was visualized by this reporter (k) or by the 10xUAS-IVS-p10-GFP reporter (i,j). The patterns seen will be referred to as those of Met and Gce in the descriptions below. (a–d) Met expression in: (a) Large polyploid larval epidermal cells, diploid histoblasts (hb), and oenocytes (oe; dotted yellow outline) at the wandering stage, (b) larval muscles, (c) larval fat body, and (d) larval salivary gland (sg) and associated fat body (fb). (e-h) Gce expression in (e) epidermis, histoblasts, and oenocytes, (f) larval muscles (g) fat body cells, and (h) larval salivary glands (sg), shown with associated fat body (fb). (i) gce-T2A-GAL4 > 10xUAS-IVS-GFP-p10 showed expression in histoblasts, epidermis, oenocytes, and larval muscles (m). (j) Gce expression was evident in all larval muscles (yellow dotted line: midline). (k) Close-up of gce-T2A-GAL4 > nls-GFP expression in larval oenocytes (dotted line) and histoblasts (hb, **). Scale bars, 50 µM.