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. 2017 May 18;7:2132. doi: 10.1038/s41598-017-02264-4

Figure 5.

Figure 5

JHR expression in larval imaginal discs. (a) Met-GAL4::p65 > mCD8-GFP expression in the eye disc, showing Met expression in the developing photoreceptors (yellow arrowheads), which show αHRP staining (a’). mCD8-GFP also shows Met expression globally in the genital disc (b) and wing disc (c) of wandering L3 animals. GFP expression was not detected in the eye (d) (αHRP staining is indicated by white arrowheads), genital (e) or wing discs (f) of wandering L3 animals, using either the gce-GAL4::p65 or gce-T2A-GAL4 drivers. The intensely-staining GFP-positive cells outlined by the white dotted line in (d) are adepithelial cells that adhere to the underside of the eye disc. (g) Quantitative PCR analysis of Met and gce expression in wing/leg discs vs. expression in CNS+ attached leg disc samples. brp serves as a CNS-specific control gene. Notum is enriched in wing discs relative to the CNS. Unpaired t-test *p < 0.05, ***p < 0.005; n = 135 for each gene: 3 technical replicates, each consisting of 3 biological replicates using material extracted from 15 animals. Error bars: SEM. (h) Normal development of the adult wing in animals fed 1 ppm dietary pyriproxyfen. Left: TM3 balancer sib control fly. Right: C765 > gce fly expressing ectopic gce in the wing disc. Arrowheads indicate the anterior margin of the adult wing. Scale bars, 50 µM.