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. 2017 May 18;7:2132. doi: 10.1038/s41598-017-02264-4

Figure 8.

Figure 8

Confocal scans of JHR expression across the adult brain, with emphasis on central complex and optic lobe expression. Myr-GFP was used to visualize JHR expression in the nervous system. Left: Two z-slices showing the overall pattern of Met expression in the adult brain (a,a’). The mushroom bodies, which show strong Met expression, are indicated with yellow arrowheads in (a); pharyngeal nerves are indicated with white arrowheads in (a’); inset: minimal expression in a group of peptidergic cells (*). (b) Met-GAL4::p65 > myr-GFP-p10 expression across the compartments of the central complex, including the ellipsoid body (EB), fan-shaped body (FB), and bulb (BU). (c) Met expression across the layers of the FB and nodulus (NO). Arrowheads indicate layers 2/3 (white) and 5 (yellow) of the FB. (d) mCD8-GFP expression and myr-GFP-p10 expression both show that Met is highly expressed in NO3 relative to other subcompartments. (e) Met-GAL4::p65  >  myr-GFP-p10 expression across the optic lobe neuropils: lobula (lo), lobula plate (lp), medulla (me), showing strongest expression in the T5 arborizations (white arrowhead) that outline the periphery of the lobula. Yellow asterisk: anterior optical tract. Center: Two z-slices showing the overall pattern of gce expression in the adult brain. The mushroom bodies (yellow arrowheads) show only weak myr-GFP expression with the gce-GAL4::p65 BAC reagent (f,f’); high level myr-GFP expression evident in the ellipsoid body and bulb (f’) with one or two yellow asterisks, respectively; (g) inner (white arrowhead) and outer (yellow arrowhead) rings of the EB show intense gce expression; (h) gce-GAL4::p65 > myr-GFP-p10 expression across FB layers is strongest in layers 4 and 6 (white arrowheads) and comparatively weak in layer 5 (yellow arrowhead). Expression was also noted in the asymmetrical body (AB), outlined with a white dotted line. (i) NO3 (white arrowhead) shows minimal GFP expression, while NO1 and NO2 show moderate expression. (j) gce expression across the optic lobe neuropils, labeled as in e. gce was absent in the arbors of the T5 cells (white arrowhead). Right: Two z-slices showing the overall pattern of gce-T2A-GAL4 > myr-GFP expression in the adult brain. Strong GFP expression is seen in the mushroom bodies (k) (yellow arrowheads) and in a subset of peptidergic cells (inset). Central complex expression is shown in (l–n); nodulus expression shows moderate to high GFP signal in NO1 and NO3 using gce-T2A-GAL4. (o) Optic lobe expression shows no GFP in the T5 cells (white arrowhead) or lobula plate (lp); medulla (me) expression is strong in some layers and weak in others. Scale bars, 50 µM. GFP (green or white), αN-cadherin (magenta).