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. 2017 May 18;7:2075. doi: 10.1038/s41598-017-02129-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of MPP⁺ and CoCl₂ on Kv3.3 and Kv3.4 expression in SH-SY5Y cells (A) RT-PCR analysis demonstrated that Kv3.3 and Kv3.4 are expressed in SH-SY5Y cells. (B) Changes in Kv3.3 and Kv3.4 mRNA expression were measured via qPCR following treatment with 1 mM MPP⁺ or 100 µM CoCl₂. Neither Kv3.3 nor Kv3.4 were affected by MPP⁺ treatment, whereas Kv3.3 was significantly decreased after 30 min, 1 h, and 24 h of CoCl₂ treatment. Kv3.4 was decreased after 4, 6, and 24 h of CoCl₂ treatment. (C) Western blotting demonstrated that 1 mM MPP⁺ did not affect Kv3.3 and Kv3.4 protein expression levels. Kv3.3 protein expression was not significantly altered by 100 µM CoCl₂ treatment, whereas Kv3.4 protein (70 kDa) expression was significantly decreased after 6 and 24 h of 100 µM CoCl₂ treatment. All experiments were performed in quadruplicate, and data represent the mean ± standard error. *p < 0.05, **p < 0.01 and ***p < 0.001 versus the control value.