Table 1.

Sequences of oligonucleotides used to clone the mSTING gene promoters and site-directed mutagenesis.

Names of plasmids	Primer sequence (5′-3′)
	Forward	Reverse
pSTING-1005	F1: CGGGGTACCTCATGCTGTCATCCACCCAGTTA	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-771	F2: CGGGGTACCCCCGGGCCTCAGCAGCTAGCTT	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-558	F3: CGGGGTACC GCCAGATGGCTAGCAGGGAAGA	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-254	F4: CGGGGTACCCAACCATCCTGAGACTGGGA	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-206	F5: CGGGGTACCAGAGTGCTCTCCCCCCTC	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-164	F6: CGGGGTACCTTCCTTGCTCAGATTTCAGG	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
pSTING-125	F7: CGGGGTACCTCTCAATCTCTCCTGTCTAACC	R1: CCCAAGCTTGCAGGACTCCATACAAGGACCAA
GATA1-mut	F8: TCCCAGTCTCAGACCGGTTGGGTACC	R8: AATTAGAGGCGTGGTTATTTCCCCGAAAAG
IK2-mut	F9: CGCCTCTAATTCTTCAGTCTCAGGAT	R9: TGGTTATTTCCCCGAAAAGAGTGCTCTC
Sp-mut	F10: GGGAAATTAGAAAAGTGGTTATTTC	R10: AGTCTCAGGATGGTTGGGTAC
STAT-mut	F11: GCGTGGTTATGGTTCCGAAAAGAG	R11: CTCTAATTTCCCAGTCTCAGGATGGTTG

Primers for cloning promoter of the mSTING gene: KpnI restriction site (underlined and italics) and protective bases were introduced to the 5′ end of the forward primers, and HindIII restriction site (underlined and italics) and protective bases were added to the 5′ end of the reverse primers. Primers for site-directed mutagenesis: the bold italics indicated the mutation sites.