Figure 5.

Obestatin stimulation is sufficient to increase slow fiber expression in skeletal muscle. (a) Western blot analysis of slow and fast MHC expression in uninjured control and freeze-injured TA muscles after intramuscular injection of obestatin (100-, 300- or 500-nmol/kg/72-h; n = 6 per time point) or vehicle (PBS) at 30 days following injury. The inserted figure shows Western blot analysis of slow and fast MHC expression in C2C12 myoblasts (GM) and myotubes obtained under DM or DM+obestatin (10 nM, 7 days). (b) Expression of the slow-fiber–specific troponin I-SS and oxidative marker myoglobin in uninjured control and freeze-injured TA muscles. In a and b protein level was expressed as fold of uninjured control TA muscles. In a and b immunoblots are representative of the mean value. In a and b, dividing lines (white lines) indicate splicing of the same gel. Full-length blots are presented in Supplementary Figure 2. Data were expressed as mean ± SEM obtained from intensity scans (*^,#p < 0.05 versus control values).