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. 2017 May 18;7:2137. doi: 10.1038/s41598-017-02337-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Obestatin signaling regulates the expression of PGC-1α and oxidative markers CPT-1, UCP-3 and Cytochrome C in skeletal muscle. Western blot analysis of PGC-1α, CPT-1, UCP-3 and Cytochrome C in uninjured control and freeze-injured TA muscles after intramuscular injection of obestatin (100-, 300- or 500-nmol/kg/72-h; n = 6 per time point) or vehicle (PBS) at 30 days following injury. Protein level was expressed as fold of uninjured control TA muscles. Immunoblots are representative of the mean value. Dividing lines (white lines) indicate splicing of the same gel. Full-length blots are presented in Supplementary Figure 4s. Data were expressed as mean ± SEM obtained from intensity scans (*^,#p < 0.05 versus control values).