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. 2017 May 18;7:2136. doi: 10.1038/s41598-017-02388-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of (somatic) variants in standard and minute primary tumor replicate samples. (A) The variant frequency in percentages of variants detected in a single replicate (1, white), in two out of three replicates (2, grey) or in all three replicates (3, black) of the two analyzed patients. Recurrent somatic variants at unique genomic positions are connected by lines to visualize variant frequency between replicates. (B) The variant frequency in percentages of somatic variants detected in a single replicate (1, white), in two out of three replicates (2, grey) or in all three replicates (3, black) of the two analyzed patients. Recurrent somatic variants at unique genomic positions are connected by lines to visualize variant frequency between replicates.