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. 2017 May 18;7:2091. doi: 10.1038/s41598-017-02286-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Daily rhythms in animals kept to a 12/12 h light/dark cycle. (A) Representative actogram of daily activity rhythm measured by means of water intake along 14 days. (B) Plasma levels of corticosterone at light/dark transition (ZT12L/ZT12D) and middle of the night (ZT18). (C) Plasma levels of melatonin at light/dark transition (ZT12L/ZT12D) and middle of the night (ZT18). (D) Daily rhythm of urinary excretion of 6-sulfatoxymelatonin (aMT6s) measured along 7 consecutive days during the day (D) and the night (N). (E) Effects of Mifepristone, a competitive antagonist of GR, in the plasma corticosterone levels measures at ZT18. The graphs show the mean ± SEM for 3–5 plasma hormone determinations or 4 urinary samples. Differences between groups were tested by Student’s t test.