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. 2017 May 18;7:2120. doi: 10.1038/s41598-017-02349-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular proteins and pro-inflammatory cytokines in broncho-alveolar lavages. BALs were sampled at 18 h.p.i. after pulmonary infection with PAO1F, CLJ1, +ExlA or −ExlA (5 × 10⁶), or mock infection with PBS, and were centrifuged to separate the cellular pellet from the soluble proteins. (a) Western blot analysis of β-actin in 3 BAL supernatants per condition (representative of 6–8 BALs per condition) and (b) histogram of band intensities. Statistics: ANOVA, p < 0.001; Holm-Sidak’s post-hoc test: *p = 0.031, **p < 0.001. (c) LDH measurements in 6 BAL supernatants per condition. Statistics: ANOVA, p < 0.001; Holm-Sidak’s post-hoc test: *p = 0.028, **p < 0.001. (d) Total proteins in BAL supernatants (n = 6). Statistics: ANOVA, p < 0.001; Holm-Sidak’s post-hoc test: *p < 0.001. (e) Myeloperoxidase activity in BAL pellets (n = 6). Statistics: ANOVA, p < 0.001; Holm-Sidak’s post-hoc test: ***p < 0.001, **p = 0.003, *p < 0.05. (f) IL-1β, TNF-α, and IFN-γ concentrations were measured in BAL supernatants (n = 6–8). Statistics: Kruskal-Wallis’s test, p < 0.001; Dunn’s post-hoc test: *p < 0.05. IL-10, IL-17 and IL-12p70 dosages yielded negligible values for all strains (not shown).