Table 3.
Row data showing positive cord blood samples as detected by qPCR, RDT and/or ELISA (n = 20).
| Subject categorization based on qPCR | Subject ID | Cord blood | Maternal blood | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| LM results | RDT results | ELISA results | ELISA mOD | ELISA Ag index | qPCR PD | Peripheral qPCR PD | Placental qPCR PD | Placental histology | ||
| qPCR positive | S44 | Neg | Neg | Neg | 0.048 | 0.2 | 150.4 | 143.8 | 22.4 | Past infection |
| S60 | Neg | Neg | Neg | 0.070 | 0.3 | 2.3 | 22.1 | 1.2 | Past infection | |
| S78 | Neg | Neg | Neg | 0.130 | 0.5 | 26.1 | 7.0 | 48.2 | Past infection | |
| S89 | Neg | Neg | Neg | 0.164 | 0.7 | 0.2 | 10150.8 | 9275.2 | Active infection | |
| S97 | Neg | Neg | Neg | 0.038 | 0.2 | 1.0 | 0.0 | 0.0 | Past infection | |
| S102 | Neg | Neg | Neg | 0.042 | 0.17 | 0.6 | 1178.0 | 39.0 | Past infection | |
| S107 | Neg | Neg | Neg | 0.063 | 0.2 | 0.3 | 256.2 | 2909.1 | Active infection | |
| S116 | Neg | Neg | Neg | 0.039 | 0.2 | 309.7 | 0.0 | NA | Active infection | |
| S127 | Neg | Neg | Neg | 0.070 | 0.3 | 0.3 | 15.0 | 1.0 | Past infection | |
| S129 | Neg | Neg | Neg | 0.051 | 0.21 | 340.5 | 111.0 | 248.5 | Active infection | |
| S151 | Neg | Pos | Pos | 3.280 | 13.45 | 5.3 | 1593.6 | 673.0 | Active infection | |
| S170 | Neg | Neg | Neg | 0.045 | 0.16 | 0.7 | 16.3 | 0.5 | Past infection | |
| S173 | Neg | Neg | Neg | 0.043 | 0.18 | 34.0 | 733.7 | 456.0 | Active infection | |
| S201 | Neg | Pos | Pos | 3.240 | 13.32 | 79.8 | 40.4 | 93.2 | Active infection | |
| S229 | Neg | Neg | Neg | 0.137 | 0.55 | 2.2 | 625.3 | 550.1 | Active infection | |
| S251 | Neg | Neg | Neg | 0.052 | 0.21 | 4.4 | 2.2 | 11.0 | Past infection | |
| qPCR negative | S33 | Neg | Pos | Pos | 3.930 | 16.6 | Neg | 1489.2 | 14341.8 | Active infection |
| S91 | Neg | Neg | Pos | 0.316 | 1.3 | Neg | 8626.0 | 13218.4 | Active infection | |
| S100 | Neg | Neg | Pos | 0.350 | 1.4 | Neg | 17998.7 | 15400.0 | Active infection | |
| S112 | Neg | Neg | Pos | 0.302 | 1.2 | Neg | 32633.0 | 5448.4 | Active infection | |
The data includes the mean optical density and the derived antigen index by ELISA, placental histology results and, parasite densities in cord, maternal peripheral and placental blood. qPCR, quantitative real-time Polymerase Chain Reaction; LM, Light Microscopy; RDT, rapid diagnostic test; ELISA, Enzyme-Linked ImmunoSorbent Assay; mOD, mean optical density; Ag index, Antigen index obtained by dividing the mOD of each sample by the cut-off value; PD, parasite density (number of parasites/µL) as calculated by qPCR; ID, identification; NA, Not available; Pos, positive (in bold); Neg, negative.